![]() It is suitable for computer-literate students (undergraduate and above) and for research staff. This record contains a self-paced tutorial to enable students to master the basic features of SciDAVis, of non-linear regression in general and to enable students to create a publication-quality figure. Commonly used pay-for software packages which carry out a similar function (and which are more powerful) include GraphPad Prism, SigmaPlot and Origin. SciDAVis is free to use and will carry out the basic analyses we need here adequately. Fitting data in this way enables us to extract parameters such as IC50 values and thus to compare the efficacy of different compounds in an experimental assay in a quantitative manner. Learn about the equation used to fit to the data.SciDAVis is a data fitting software package used to fit physically meaningful equations to experimental (assay) data using non-linear regression.At the bottom of the dialog, check the option to "Interpolate unknowns from standard curve". response - Variable slope (four parameters)". On the Nonlinear regression dialog, open the "Dose-Response - Inhibition" family of equations, and choose "log(inhibitor) vs.Or click the Nonlinear Regression shortcut button just above the Analyze button. Click Analyze and then Nonlinear regression.These are Y values without corresponding X values. Also note that this sample data set includes unknown values. ![]() Be sure you select the correct equation when performing nonlinear regression! If you'd like to convert concentration values to log(concentration) values - or vice versa - you can use Prism's Transform analysis to convert the X values. Prism offers built-in equations designed to handle X values as either concentration OR log(concentration). Note that the X values are logarithms of concentration.From the Welcome dialog, choose the XY tab, drop the list of sample data sets and choose "RIA or ELISA".Prism can easily fit a dose response curve to determine the IC 50. ![]()
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